The Akatsuki is a criminal organization that have acted in shadow in every season of Super Smash Bros. Crossover. After Sasuke Uchiha is arrested at the end of Season 19, the Akatsuki serves as the primary antagonistic force of Season 20. After Season 20, the group is dissolved, with most of the members being dead while the others have defected. The Akatsuki is reborn in Season 22 due to the rift caused by the improper revival of The Offspring of Zorc.
Tobi - The leader of the Akatsuki, who desires world domination. Zetsu - A member of the Akatsuki that acts as their spy. Zetsu is the right hand man of Tobi.
Nagato - Nagato was the second in command of the Akatsuki, until Tobi betrayed him and Konan.
Konan - The first female member of the Akatsuki, who was betrayed by Tobi.
Deidara - An Akatsuki member who relies on explosive clay to attack.
Sasori - An Akatsuki member who is a skilled puppet master.
Hidan - An Akatsuki member who is immortal, but can die from lack of nutrients.
Kakuzu - An Akatsuki member who has several hearts, making him impossible to kill.
Kisame Hoshigaki - An Akatsuki member who is one of the Seven Swordsman of the Mist.
Orochimaru - Defected prior to the start of the series.
Itachi Uchiha - A member of the Akatsuki that secretly helped the heroes in order to protect Sasuke. Itachi's cover is later blown during Season 19, and is no longer a member of the organization, though he kept his ring so he can't be replaced.
Pandoria - Left the organization following Samus' defeat.
Infinity Mecha Sonic - Left the organization after Samus is defeated.